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Studies:

• Chemical Constituents / Triterpenoids / Flavonoids: (1) Study isolated four compounds from the n-hexane and chloroform fractionates: 3,5,7-trihydroxy-3'4'-dimethoxy flavone, betulinic acid (dillenetin), ß-sitosterol and stigmaterol. Results indicate Dillenia indica may provide a rich source of triterpenoids and flavonoids. (2) Contains the lupeol group of triterpene (betulinic acid, betulinaldehyde, betulin) and flavonol (myricetin). Stem bark contains myricetin, isorrhamnetic, dillenetin and glucosides.
• Betulinic Acid / Anti-Leukemic: The methanolic extract of Dillenia indica fruit showed significant anti-leukemic activity in human leukemic cell lines. Betulinic acid, the major compound isolated, could explain the anti-leukemic activity.
• Anti-Inflammatory: The anti-inflammatory activities of the methanol extract of Dillenia indica leaves were observed in various models related to inflammation. The findings support the folkloric use of Dillenia indica in diseases related to inflammatory processes.
• Antioxidant: Study of extracts of Dillenia indica fruits antioxidant activity to be highest in the methanol extract, followed by ethyl acetate and water extracts. Results indicate the extent of antioxidant activity correlated with the amount of phenolics present and that D. indica is rich in phenolics and may provide a good source of antioxidants.
• Anti-Diabetic / Antihyperlipidemic: Study of methanolic leaves extracts in STZ-induced diabetic Wistar rats showed significant anti-diabetic activity. It also showed significant reduction in serum cholesterol, triglycerides, and serum transaminases levels, with improvement in HDL levels. Extract treatment also enhanced serum insulin levels in diabetic rats.
• Antimicrobial: Study of methanolic extracts and fractions of the bark of DI showed remarkable activities against all test bacteria. An n-Hexane fraction showed highest activity against Shigella dysenteriae. A methanol extract showed highest activity against fungus Candida albicans.
enhanced serum insulin levels in diabetic rats.
• Antimicrobial / Antioxidant / Cytotoxicity: Crude methanolic extracts showed weak antimicrobial activity against bacteria and fungi. Extractives exhibited significant cytotoxic activity on brine shrimp lethality bioassay. Extractives also exhibited significant free radical scavenging activity.
• Anxiolytic: Study of a hydroethanolic leaves extract showed prominent anxiolytic activity in mice. Diazepam was used as the standard drug.
• Free Radical Scavenging Activity: Study of methanolic leaves extract showed significant reducing power and concentration-dependent free radical scavenging effect. Total phenolic contents of the leaves extract were gallic acid equivalents and total flavonoids were catechin equivalents.

Introduction: Ingredients of Indian cuisine are well known for their antioxidant properties which can help prevent many diseases. The fruits of Dillenia indica and Garcinia penducalata are commonly used by the Assamese speaking population of Assam, India, but their antioxidant properties have not been investigated. Methods: Methanol, petroleum ether and water extracts of the shade dried fruits of Dillenia indica and Garcinia penducalata were obtained and the IC50 values of their DPPH, hydroxyl, oxygen and nitric oxide scavenging activities were estimated along with their reductive ability, vitamin C and total phenolic content. Vitamin C was used as a standard reference for the antioxidant scavenging activities. Result: The IC50 values for DPPH, hydroxyl, oxygen and nitric oxide inhibition of vitamin C were 43.7 ug/ml, 50.44 ug/ml, 61.04 ug/ml and 41.82 ug/ml, respectively while the IC50 value for its reductive ability was 47.17 ug/ml. The IC50 values for the DPPH, hydroxyl, oxygen, nitric oxide and reductive ability of the methanolic extract of Dillenia indica were 31.25 ug/ml, 51.82 ug/ml, 51.44 ug/ml, 39.73 ug/ml and 40.18 ug/ml, respectively. These IC50 values were superior in comparison to its petroleum ether and water extracts. The methanolic extract of Garcinia penducalata had the highest amount of phenolic content and its IC50 values for DPPH, oxygen and nitric oxide scavenging activities were 50.23 ug/ml, 66.06 ug/ml and 63.02 ug/ml, respectively.

Conclusion: The higher amount of phenolic content in the methanolic extract of Dillenia indica may contribute to its better in vitro anti oxidant property.

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