Prolistem - New Azoospermia Treatment

For decades, the traditional approach for men with non-obstructive azoos***mia has focused on hormone therapy, often followed by microTESE.

But we began asking an important question: If a man already has elevated hormone levels, such as FSH or LH, why should we give him even more hormonal stimulation?

Imagine a flower growing in a pot filled with too much water. If the flower begins to die because it has been overwatered, adding more water will not help. The solution is to improve drainage, remove the excess water, and restore a healthier environment in which the flower may grow again.

Prolistem was developed around a similar concept. Instead of adding more hormonal stimulation, its strategy is designed to temporarily reduce excessive intratesticular testosterone signaling and create a more supportive testicular environment for the cells involved in s***m production.

By improving the cellular environment, Prolistem aims to give the remaining s***matogenic cells a better opportunity to progress through the s***m-development cycle—before moving to microTESE.

This is why Prolistem opens new hope for men with azoos***mia: it introduces a different strategy where traditional approaches have offered limited options.

Prolistem is not a guaranteed cure, and outcomes vary between patients. However, it provides a new scientific direction for helping selected men with non-obstructive azoos***mia. ***mia

Managing Non-Obstructive Azoos***mia

In men with non-obstructive azoos***mia caused by primary testicular failure, the problem is s***m production not a blockage.

When s***m are not found in the semen, international guidelines recommend microTESE as the preferred surgical method for retrieving s***m for ICSI.

Hormonal treatments such as hCG may help men with specific hormonal deficiencies. However, in primary testicular failure, evidence that hormonal therapy significantly improves microTESE outcomes remains limited.

The Prolistem strategy begins before surgery.

Prolistem was developed from our research concept at MD Anderson Cancer Center: support the testicular environment and give the remaining s***m-producing cells an opportunity to continue and complete the s***matogenesis cycle.

Our proposed management pathway is:

Six months of Prolistem → regular semen analysis → microTESE if s***m are not found

If s***m appear in the semen, they may be collected and used for ICSI. If no s***m are detected after six months, the patient can proceed to microTESE as the next step.

Prolistem does not guarantee s***m production or retrieval. It provides a new pre-microTESE strategy for selected men with non-obstructive azoos***mia.

Clinical Evidence for Prolistem in Non-Obstructive Azoos***mia (NOA)

Two clinical studies evaluated Prolistem in men with NOA and previous unsuccessful s***m-retrieval procedures.

2024 Controlled Study – 77 patients
S***m retrieval during microTESE:
54.5% with Prolistem vs. 21.2% in the control group

2026 Retrospective Study – 29 difficult cases with ≥2 previous failed microTESE procedures
10.3% had s***m detected in semen after treatment. Among patients who underwent repeat microTESE, 23% had successful s***m retrieval.

These findings support continued clinical research into Prolistem as a potential strategy for selected men with challenging NOA.

Results vary between patients and do not guarantee s***m production, retrieval, pregnancy, or live birth.

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Messages like this truly warm our hearts. ❤️

Sometimes, we don’t even know about the success stories happening after Prolistem treatment until a message like this reaches us.

Knowing that our work has helped bring hope to a family, and contributed to the journey toward welcoming a child, means more to us than words can express.

Thank you for sharing your stories with us. Every success gives us even more motivation to keep going. 🙏
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Prolistem Treatment Pathway for Non-Obstructive Azoos***mia

Ask these important questions about your azoos***mia:

• What type of azoos***mia do I have?
• What is the likely cause?
• What do my FSH, LH and testosterone levels tell me?
• Do I need genetic testing?
• What are my chances of finding s***m?
• Should I prepare before microTESE?
• Are there options I should consider before surgery?

Azoos***mia is complex. Understanding your individual case is the first step toward choosing the right strategy.

At Prolistem, we are happy to discuss your case and help guide you through your journey

Can the temporary reduction in testosterone with Prolistem cause side effects?

This is one of the most common questions we receive.

The answer begins with understanding where testosterone works.

Most of the testosterone in the body circulates in the bloodstream. However, inside the te**is, testosterone levels are naturally much higher and play a key role in regulating the environment surrounding the s***m-producing stem cells.

During Phase 1, Prolistem is designed to induce a temporary and controlled reduction in intratesticular testosterone—not complete testosterone suppression. This modest change is intended to help “reset” the signaling environment within the seminiferous tubules, particularly the activity of Sertoli cells, which support s***m development.

Why is this important?

Research has shown that carefully reducing intratesticular testosterone for a limited period can stimulate biological pathways involved in s***matogonial stem cell activation and regeneration. This concept has also been explored in experimental studies investigating the recovery of s***matogenesis.

In our clinical experience, this temporary reduction has been well tolerated, and testosterone levels are restored during Phase 2, when the focus shifts to supporting s***m maturation.

At Prolistem, every step of the treatment strategy has a purpose—because improving the testicular environment comes before improving the chances of finding s***m.

How does Prolistem support men with azoos***mia?

Prolistem is designed to help improve the biological environment inside the te**es—an environment largely regulated by Sertoli cells.

Sertoli cells are often called the “nurse cells” of s***matogenesis because they protect, nourish, and support germ cells throughout their development into mature s***m.

In our research, we observed that s***matogonial stem cells obtained from men with azoos***mia were still able to survive and grow when placed in a more supportive environment outside the te**es. This suggests that, in some patients, the stem cells may retain developmental potential, while the surrounding testicular environment—particularly Sertoli-cell support—may be impaired.

This finding supports Prolistem’s strategy: rather than focusing only on the germ cells, the program aims to support and improve the Sertoli-cell-controlled environment required for s***m development.

Prolistem targets the environment that supports s***matogenesis—helping create better conditions for s***m production before microTESE.

Results may vary between patients. Prolistem is intended for selected men with non-obstructive azoos***mia under medical supervision.

Are you suffering from azoos***mia?

Not all azoos***mia is the same.

Prolistem® is specifically developed for men with non-obstructive azoos***mia (NOA), also known as primary testicular failure.

Our primary goal is to increase the chances of successful s***m retrieval during microTESE in carefully selected patients.

Clinical experience has also shown that approximately 15% of treated patients may have s***m detected in their ej*****te, potentially avoiding the need for surgical s***m retrieval.

Every patient is unique, and proper diagnosis and evaluation by a fertility specialist are essential to determine the most appropriate treatment strategy.

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How to Make Sure Your Prolistem is 100% Original
Your health and journey are too important to risk on counterfeit products. To ensure you are getting the genuine, scientifically formulated Prolistem, always verify your purchase using our official channels.
Here is how you can guarantee your Prolistem is the real deal:
Buy Directly from the Source: The safest way to purchase is through our only official website: prolistem.com.
Check Our Authorized Partners: We work with a select network of trusted distributors. You can find the fully updated list of official distributors directly on our website’s dedicated page.
Verify with Us Directly: If you have already purchased a box or are unsure about a seller, we are here to help. Reach out to our team to verify authenticity before you start your regimen:
Phone: +1 646 833 9791
Email: [email protected]