JN Beauty Solutions

GLYCINE
AMINO ACID FOR ANTI-AGING & CELLULAR METABOLISM
Presented by Jn Beauty Solutions®

In the world of supplements and anti-aging therapies, Glycine stands out as a “silent hero.” As the simplest amino acid in structure, glycine participates in most of the key processes in the body: from building collagen to regulating sleep, providing powerful antioxidant effects, and activating the cellular self-cleaning mechanism (autophagy).

Not only is it a “building material,” glycine is also a molecule that regulates the entire biological system, especially when combined with N-acetylcysteine (NAC) in the GlyNAC formula — one of the most promising anti-aging therapies available today.

This article provides a comprehensive overview, based on scientific evidence, of Glycine: from its history and mechanisms to its practical applications (oral and intravenous).

1. OVERVIEW OF GLYCINE
Glycine (C₂H₅NO₂, MW: 75.07 g/mol) is the simplest amino acid among the 20 amino acids that make up proteins, with an R-group consisting of only one hydrogen atom.

Although classified as a non-essential amino acid, Glycine often becomes conditionally essential in many physiological and pathological situations, when demand exceeds the body’s endogenous synthesis capacity (in older adults, high oxidative stress, metabolic disorders).

Core Biological Roles:
• Main component of collagen (~35%).
• Precursor for glutathione (GSH) – the body’s most powerful endogenous antioxidant.
• Participates in one-carbon metabolism.
• Inhibitory neurotransmitter in the central nervous system.
• Regulates sleep, inflammation, and aging.
Glycine is not only a “building material” but also a molecule that regulates the entire biological system.

2. RESEARCH HISTORY
• 1820: Henri Braconnot (France) isolated glycine from gelatin.
• 1838–1848: Confirmed to contain nitrogen; Jöns Jacob Berzelius named it “glycine.”
• 1858: Auguste Cahours determined its structure.
• 1953: The Miller-Urey experiment proved that glycine could form naturally under prebiotic conditions.
• 2000s–present: Focused research on GlyNAC (anti-aging), neurological and psychiatric roles, metabolism, and healthy lifespan extension.

3. BIOLOGICAL MECHANISMS OF ACTION
3.1 Structural Role – Collagen
Glycine occupies repeating positions in the Gly–X–Y chain, allowing the formation of a stable triple helix – the foundation for the durability of skin, tendons, joints, and connective tissues.

3.2 Enhanced Antioxidant Defense – Glutathione
Glycine is an essential component for GSH synthesis. When combined with NAC (which provides cysteine) in GlyNAC, GSH increases by ~164%, oxidative stress decreases by ~70–72%, and mitochondrial function improves significantly.
3.3 Neurological Regulation
- Acts as an inhibitory neurotransmitter via the glycine receptor (GlyR).
- Co-agonist of the NMDA receptor → supports memory, learning, and mental stability.

3.4 Sleep Regulation
Acts on the SCN region (biological clock), causing peripheral vasodilation → lowers core body temperature → increases deep sleep (slow-wave sleep) and reduces daytime fatigue.

3.5 Detailed Anti-Aging Mechanisms
GNMT Pathway (Methionine Restriction Mimetic) Glycine activates the enzyme GNMT → reduces excess methionine/SAM → decreases mTOR activation → increases autophagy and mitophagy, supporting cellular regeneration and clearance of damaged proteins. Sarcosine (a product of GNMT) also directly activates autophagy.

GlyNAC – Powerful Anti-Aging Formula Glycine + NAC:
- Restores endogenous GSH.
- Reduces inflammation (IL-6, TNF-α), insulin resistance, and DNA damage.
- Improves muscle strength, cognition, mitochondrial function, and multiple hallmarks of aging.

Systemic Effects Increases cellular energy, protects vascular endothelium, supports stem cells, and improves healthspan (healthy lifespan).

4. MAIN CLINICAL BENEFITS
🧡Sleep: Reduces time to fall asleep, improves deep sleep quality, and decreases daytime fatigue.
🧡Anti-Aging: Increases autophagy, restores mitochondria, and reduces oxidative stress.
🧡Skin – Collagen – Joints: Increases collagen synthesis, improves skin elasticity, and supports joints.
🧡Metabolism: Increases insulin sensitivity, reduces triglycerides, and supports liver health.
🧡Neurological: Supports schizophrenia treatment (high doses) and improves cognition.
🧡Cardiovascular & Recovery: Reduces inflammation, protects endothelium, and enhances muscle recovery.

5. CLINICAL EVIDENCE
👉Sleep: Studies by Yamadera (2007) and Bannai (2012) – 3g glycine before bed significantly improves sleep quality (polysomnography).
👉GlyNAC: RCTs show GSH increase of 164%, oxidative stress reduction of 72%, and improvements in muscle strength, cognition, and multiple hallmarks of aging.
👉Psychiatric: Doses of 15–60g/day improve negative symptoms of schizophrenia.
👉Others: Benefits in metabolism and stroke recovery.

6. INTRAVENOUS GLYCINE APPLICATIONS
👉Role of IV Route:
Bypasses first-pass metabolism, rapidly increases plasma concentration, and provides higher efficacy for detoxification, acute antioxidant support, and recovery.

👉Potential Applications:
• Strong antioxidant effects (rapid GSH increase).
• Liver support (fatty liver), neurological health, and recovery from oxidative stress.
• Anti-aging IV protocols, mitochondrial therapy, and detox IV.

👉Comparison of Oral vs IV Feature Oral IV Bioavailability Moderate Very High Onset of Action Slow Fast Applications Sleep, maintenance Detox, anti-aging, recovery Dose Control Low Precise

👉Reference Dosages (only under medical supervision):
• Basic: 0.5–2g IV, infused slowly over 30–60 minutes.
• Advanced (anti-aging/detox): 1–3g, combined with Glutathione, Vitamin C, Magnesium, NAC.
• Surgical: 1.5% solution for endoscopic irrigation.

7. OVERALL RECOMMENDED DOSAGE
• Oral: Sleep – 3g before bedtime; Anti-aging – 5–15g/day (divided into 1–3 doses).
• IV: 0.5–2g (basic) or 1–3g (advanced protocols), as prescribed by a physician.

8. SAFETY PROFILE
Glycine is very safe at standard doses. Side effects are rare and mild (nausea or mild digestive discomfort with high oral doses).
Notes: Do not use with clozapine. Use with caution in pregnant women or patients with severe psychiatric conditions. IV administration should only be performed in a medical setting.

9. OPTIMAL COMBINATION RECOMMENDATIONS
• Anti-Aging: Glycine + NAC (GlyNAC).
• Sleep: Magnesium glycinate.
• Skin & Collagen: Collagen peptides + Vitamin C.
• Energy: Creatine + Omega-3.

Glycine is a small molecule but plays an extremely important foundational role in biology, tightly connecting structure (collagen), antioxidant defense (GSH), neurology (GlyR, NMDA), and anti-aging (GNMT + autophagy + GlyNAC).

With increasingly strong scientific evidence, especially in GlyNAC therapy, Glycine is becoming a “core molecule” in modern longevity and metabolic optimization strategies.

Professional Recommendations: Oral use is suitable for long-term application for sleep and maintenance. Intravenous administration should be performed in a medical environment to achieve optimal efficacy and safety. Personalize dosage and protocols according to your specific health goals.

JN Beauty Solutions®
Beauty From Science

THE LIFE SUSTAINING TECHNOLOGY
JN-LSS2020 - JN Life Sustaining System

🔵SUMMARY
At JN Beauty Solutions™, we believe that pure, natural ingredients and scientifically proven therapies form the core foundation of professional skincare. With over 20 years of experience in research, development, and application of cosmeceuticals, we always prioritize combining superior, stable, and clinically supported active ingredients into our products to deliver real, safe, and long-lasting results.

JN-LSS2020 (Life Sustaining System) – the Skin Life Sustaining Technology – is a groundbreaking research achievement built upon advanced Nano Transdermal Delivery Technology combined with Active Plus Advanced Formulae. This technology creates a comprehensive skin nourishment system that repairs damaged skin structures at the cellular level, maintains skin vitality, and enhances the skin’s long-term self-repairing capacity.

The technology is specifically optimized for Fitzpatrick Skin Types III–IV – the most common skin types among Asian populations and medium to dark skin tones.

The Fitzpatrick Skin Type Scale is the international gold standard developed by Dr. Thomas B. Fitzpatrick in 1975. It classifies skin based on natural skin color and its reaction to ultraviolet (UV) radiation. Skin Types III–IV tan easily but are highly prone to hyperpigmentation, melasma, and post-inflammatory pigmentation caused by UV exposure (photoaging). JN-LSS2020 has been validated through thousands of real-world cases since 2020, delivering clear clinical results in treating melasma due to aging, skin rejuvenation, texture improvement, and wrinkle reduction, particularly in this skin type group.

🔵TREATMENT PHILOSOPHY OF JN BEAUTY SOLUTIONS®
JN Beauty Solutions® is a pioneer in applying the comprehensive skincare treatment cycle “Nourish – Restore – Treat – Improve – Nourish” since the early 2000s.

We believe that damaged skin is like a body that is starving and thirsty. One cannot force aggressive treatment when the skin lacks sufficient strength. Therefore, we have developed the following logical treatment cycle:

• Nourish: Supply essential nutrients (Amino Acids, Vitamins, Stem Cell Extracts…) to provide skin cells with energy and a solid foundation.
• Restore: Repair the skin barrier, regenerate intercellular lipids, and reduce chronic inflammation.
• Treat: Use potent targeted actives (Tranexamic Acid, Niacinamide, MAP Vitamin C…) to address the root causes of skin concerns.
• Improve: Activate strong regeneration to deliver visibly brighter, smoother, firmer skin with reduced wrinkles and even skin tone.
• Nourish Long-term: Continuously maintain skin health, enhance self-repair capacity, and prevent recurrence.

The core spirit of JN-LSS2020: Nourishment is the foundation, restoration is the key, treatment must be applied at the right time, and long-term maintenance is the ultimate goal.

🔵METHODOLOGY & OBJECTIVES
JN-LSS2020 is formulated based on the Natural Ingredients Base – a blend of highly pure ingredients with superior pe*******on capability. Thanks to the Nano Transdermal Delivery system, active ingredients rapidly pe*****te into the dermis without leaving any greasy or sticky residue on the epidermis. This system minimizes trans epidermal water loss (TEWL) and ingredient waste, ensuring optimal efficacy, economical use, and reduced risk of irritation.

Scientific studies indicate that approximately 80% of aesthetic dermatological concerns (pigmentation disorders, collagen deficiency, aging, inflammation, etc.) stem from overall or localized skin aging, especially photoaging caused by cumulative UV exposure. JN-LSS2020 applies the principle of addressing root causes while simultaneously treating symptoms, delivering faster visible results and effectively preventing recurrence — especially pigmentation disorders and chronic inflammation in Fitzpatrick Skin Types III–IV.

The technology delivers a carefully selected blend of Amino Acids, Peptides, Stem Cell Extracts, Vitamins, and Minerals, combined with targeted actives for specific concerns:
• Pigmentation disorders: Tranexamic Acid, Alpha Arbutin, Kojic Acid…
• Inflammation: Niacinamide, Bio-Sulfur, Vitamin B…
• Multi-layer aging: Hyaluronic Acid, Matrixyl®3000, Argireline®, Stem Cell Extract, DNA…
The entire blend is extracted from natural sources using Advanced Extraction Technology and Nano Transdermal Delivery, creating a powerful synergistic effect that is both safe and fast-acting.

🔵DETAILED PRINCIPLES AND MECHANISM OF ACTION
JN-LSS2020 operates based on the principle of “Maintaining & Restoring Skin Cell Life” through three simultaneous layers:
Layer 1. Deep and Sustained Pe*******on
Layer 2. Multi-Layer Synergistic Action (Synergistic Multi-Action)
Layer 3. Root-Cause Restoration & Recurrence Prevention

🌀1. Core Principle:
Natural Ingredients Base + Nano Transdermal Delivery The system uses Phosphatidyl Choline (PC) as the primary carrier, combined with Nano technology (

NEUROWAVE™
DSIP - Delta Sleep-Inducing Peptide

🔶ABOUT NEUROWAVE™
NEUROWAVE™ is the trade name of a peptide line researched and introduced by JN Beauty Solutions®. NEUROWAVE™ is an advanced neuro-regulatory solution utilizing Delta Sleep-Inducing Peptide (DSIP) – an endogenous neuropeptide – to optimize deep sleep and support nervous system recovery through natural biological mechanisms.

Unlike traditional sleep-inducing methods (sleeping pills, sedatives), NEUROWAVE™ does not suppress the central nervous system or induce forced drowsiness. Instead, it helps restore the biological rhythm of sleep, regulates the neuroendocrine system, and promotes brain recovery during the deep sleep phase (slow-wave sleep). This represents a new approach in the field of NEURO-REGENERATION & SLEEP OPTIMIZATION.

🔶RESEARCH HISTORY
DSIP (Delta Sleep-Inducing Peptide) was discovered in the 1970s during studies on slow-wave sleep. This peptide was first isolated from animal brain tissue and later identified in human cerebrospinal fluid (CSF), showing a direct connection to delta wave activity – the deepest stage of sleep.

Over more than 40 years of research, DSIP has been regarded as a neuro-regulatory neuropeptide involved in sleep, stress, and endocrine balance. Unlike symptomatic treatments, DSIP is approached as a natural biological modulator.

🔶WHAT IS DSIP PEPTIDE?
DSIP is an endogenous neuropeptide consisting of 9 amino acids, primarily researched for its role in regulating deep sleep (slow-wave sleep) and supporting neuroendocrine balance. Unlike conventional sleep aids, DSIP does not directly induce drowsiness but helps the brain transition into a proper, deeper sleep state.

🔶BIOLOGICAL NATURE OF DSIP PEPTIDE
DSIP belongs to the group of regulatory neuropeptides, present in cerebrospinal fluid (CSF), and is directly linked to delta brain waves – characteristic of the deep sleep phase (slow-wave sleep, including stages 3 and 4). This is the most critical phase for nervous system recovery, cellular regeneration, hormone regulation, and clearance of brain waste.

🔶MECHANISM OF ACTION
NEUROWAVE™ operates through key biological axes (based on preclinical and preliminary clinical studies):

🔆1/ Regulation of the HPA Axis (Stress Axis Regulation)
Reduces nighttime cortisol levels
Reduces brain "hyper-alert" state
Reduces mid-night awakenings
→ Helps the body easily transition to a deep resting state.

🔆2/ Balance of Neurotransmitter Systems
Supports GABA activity (relaxation)
Modulates glutamate (excitation)
→ Creates ideal conditions for the brain to enter deep sleep phases.

🔆3/ Optimization of Delta Brain Waves (Deep Sleep Architecture)
Increases duration of slow-wave sleep (SWS)
Improves depth and continuity of sleep
→ Supports: Neural regeneration | Hormone recovery | Cellular repair

🔆4/ Neuroendocrine Regulation
Influences ACTH and cortisol
Supports balance of the neuroendocrine axis

🔶CLINICAL EVIDENCE
Clinical studies (primarily from the 1980s, small-scale, double-blind) indicate DSIP's potential to improve sleep quality:
👉Clinical Trial – Insomnia (Double-blind): Some studies reported increased sleep efficiency, reduced sleep latency, and improved morning recovery sensation. However, efficacy was rated as weak in some cases and not always superior to placebo.

👉Clinical Study – Short-term Protocol (7 days): Improved sleep quality, increased daytime performance, with effects maintained in some subjects after discontinuation.

👉Biomarker Evidence (CSF Studies): DSIP is directly linked to deep sleep (stages 3/4) and delta wave activity, confirming its biological role in sleep structure.

👉Scientific Reviews: Comprehensive reviews suggest DSIP may increase slow-wave sleep, regulate cortisol, and reduce neural stress in certain subjects, though results remain conflicting and require larger-scale studies.

🔶KEY BENEFITS (BASED ON RESEARCH AND REPORTS)
- OPTIMIZES DEEP SLEEP (INCREASES SLOW-WAVE SLEEP)
- DEEPER SLEEP WITH FEWER INTERRUPTIONS AND MIDNIGHT AWAKENINGS
- IMPROVES POST-SLEEP RECOVERY QUALITY
- REDUCES BASELINE STRESS AND NIGHTTIME CORTISOL
- REDUCES SLEEP-RELATED ANXIETY
- NON-ADDICTIVE
- DOES NOT SUPPRESS THE CENTRAL NERVOUS SYSTEM
- NO "SLEEP HANGOVER" (EXCESSIVE DAYTIME DROWSINESS)

🔶APPLICATION VALUE
NEUROWAVE™ is particularly suitable for:
 Stress-induced insomnia
 Shallow sleep with frequent awakenings
 Sufficient sleep duration but no feeling of restoration
 Individuals seeking to optimize brain performance and recovery

🔶USAGE INSTRUCTIONS (PROFESSIONAL PROTOCOL – FOR RESEARCH USE ONLY)
🔆Principles:
Administer in the evening before bedtime
Not to be used as an immediate sleep aid
Apply in courses, personalized

🔆Reference Dosage (based on peptide research):
100 – 300 mcg per dose
Daily or EOD (every other day)

🔆Suggested Application Protocols:
Neuro Recovery Protocol
Deep Sleep Optimization Program
Stress Reset Therapy

🔆Common Combinations:
Magnesium (injection or infusion)
Glycine (injection or infusion)
Physiological-dose Melatonin (oral)

🔶PRECAUTIONS
- Does not replace medical treatment for pathological insomnia (chronic insomnia requires a physician).
- Not for inducing immediate sleep.
- Dosage must be personalized under professional guidance.
- Start with low dose and titrate gradually to monitor response.
- DSIP is a research peptide, not widely approved for insomnia treatment.

🔶SCIENTIFIC POSITIONING
NEUROWAVE™ is not:
A sleeping pill
A sedative
A CNS depressant

NEUROWAVE™ is:
Advanced Neuro-Regulation System for Sleep Recovery

NEUROWAVE™ – DSIP Advanced Sleep Recovery represents a new approach to sleep care and neural recovery:
✔ Physiological – no forcing
✔ Regulatory – no suppression
✔ Restorative – no dependence

NEUROWAVE doesn't just help you sleep…
It helps your body restore the natural ability to sleep properly.

NEUROWAVE doesn't help you fall asleep faster…
It helps you sleep deeper, more rhythmically, and recover better.

For more details about the product, please contact:
JN BEAUTY SOLUTIONS®
Beauty From Science

TRIPLE REJUVENATES SERUM 2026
Advanced Peptide Rejuvenating Regeneration Technology

🟡GENERAL INFORMATION
After more than four years on the market and being trusted by professional spa systems, aesthetic clinics, and skincare experts, the TRIPLE REJUVENATES SERUM line from JN Beauty Solutions® has become one of the most effective skin rejuvenation solutions, thanks to its advanced peptide-based skin structural regeneration technology.

Entering 2026, JN Beauty Solutions® officially introduces TRIPLE REJUVENATES SERUM 2026 — a comprehensive upgrade of the brand’s well-known peptide rejuvenation formula.

This upgraded version was developed to optimize skin rejuvenation performance based on the latest peptide research, addressing modern aesthetic skin concerns such as:
- Premature Aging
- Collagen Depletion
- Oxidative Aging Stress
- Loss Of Skin Elasticity
- Dynamic Wrinkles
- Static Wrinkles

At the same time, the 2026 edition also marks a strategic partnership with a new distributor in the Vietnamese market, where the demand for scientific and advanced cosmeceutical skincare solutions is rapidly growing.

TRIPLE REJUVENATES SERUM 2026 is developed based on the philosophy of JN Beauty Solutions®:
NOT ONLY CARING FOR THE SKIN — BUT ACTIVATING THE REGENERATION AND RESTORATION OF SKIN STRUCTURE AT THE CELLULAR LEVEL.

🟡REJUVENATING PEPTIDE REGENERATION TECHNOLOGY
The highlight of TRIPLE REJUVENATES SERUM 2026 lies in the combination of three premium bioactive peptides, considered one of the most advanced skin rejuvenation peptide systems in scientific skincare.

The three key active ingredients include:
 Acetyl Hexapeptide-8 (Argireline)
 Palmitoyl Tetrapeptide-7 (Matrixyl® peptide complex)
 Tripeptide-29
These peptides work through three different rejuvenation mechanisms, creating a powerful Triple Rejuvenation System.

🟡KEY BIOACTIVE INGREDIENTS
➡️Acetyl Hexapeptide-8 (Argireline)
Expression Line Relaxing Peptide

Argireline is widely recognized as one of the most famous anti-wrinkle peptides in aesthetic skincare, often referred to as a “Botox-like peptide” in cosmetic formulations.

Mechanism of action:
• helps reduce micro-muscle contractions on the skin surface
• limits the formation of expression lines
• softens common wrinkle areas such as the forehead, crow’s feet, and smile lines

➡️Palmitoyl Tetrapeptide-7 (Matrixyl® Peptide Complex)
Collagen Repair & Skin Structure Peptide

Palmitoyl Tetrapeptide-7 is part of the well-known Matrixyl® peptide system, widely studied in anti-aging technologies.
This ingredient helps:
• stimulate collagen and elastin production
• support the repair of the skin’s structural matrix
• improve skin firmness and elasticity
• In addition, this peptide helps reduce micro-inflammatory signals in the skin, one of the key contributors to premature aging.

➡️Tripeptide-29
Advanced Collagen Stimulating Peptide

Tripeptide-29 is a premium bioactive peptide used in advanced skin rejuvenation formulations. This peptide plays an important role in enhancing deep skin structural regeneration.
It helps:
• stimulate natural collagen synthesis
• improve dermal structural density
• gradually smooth deeper wrinkles over time

➡️HYDRATION SUPPORT SYSTEM
In addition to the rejuvenating peptide complex, the formula is supported by a biological hydration system that helps optimize peptide performance. Key components include:

➡️Hyaluronic Acid & Sodium Hyaluronate
• provide deep skin hydration
• help fill fine lines
• improve skin softness and smoothness

➡️Arginine Alpha-Ketoglutarate
• supports cellular energy metabolism
• promotes skin recovery and regeneration

🟡INTELLIGENT PEPTIDE SYNERGY
The effectiveness of TRIPLE REJUVENATES SERUM 2026 does not come solely from individual ingredients, but from the synergistic interaction between the active peptides.

 Argireline helps relax expression wrinkles
 Matrixyl peptide complex supports collagen structural regeneration
 Tripeptide-29 stimulates deeper collagen synthesis

Together, these mechanisms create a Triple Rejuvenation System:
1️⃣ smoothing the skin surface
2️⃣ regenerating skin structure
3️⃣ improving collagen density

🟡OVERALL BENEFITS OF TRIPLE REJUVENATES SERUM 2026
✔ Reduces expression wrinkles
✔ Improves skin firmness and elasticity
✔ Stimulates collagen production
✔ Restores aging skin structure
✔ Enhances skin smoothness and vitality

🟡INGREDIENTS
Aqua, Propanediol, Ethoxydiglycol, Glycerin, Hyaluronic Acid, Sodium Hyaluronate, Arginine Alpha-Ketoglutarate, Acetyl Hexapeptide-8 (Argireline), Palmitoyl Tripeptide-1, Palmitoyl Tetrapeptide-7 (Matrixyl 3000), Tripeptide-29, Sodium Phytate, Sodium Citrate, Phenoxyethanol, Citric Acid…

🟡DIRECTIONS FOR USE
Apply 3–5 drops of the serum and gently massage into aging areas or the entire face until fully absorbed after cleansing with an appropriate facial cleanser. Follow with a suitable moisturizer. Use twice daily for optimal visible results.

This product may be used for home skincare routines or as a finishing serum following aesthetic procedures such as:
- laser treatments
- microneedling
- RF microneedling
- other non-invasive aesthetic treatments.

TRIPLE REJUVENATES SERUM 2026 represents a new advancement in the peptide skin rejuvenation technology of JN Beauty Solutions®. By combining advanced peptides with a biological hydration system, the formula delivers a multi-mechanism and comprehensive skin rejuvenation solution.

This formulation is designed not only to improve visible signs of aging, but also to restore the natural health and structural integrity of the skin from within.

With this upgraded version, JN Beauty Solutions® continues to reaffirm its philosophy:
✔ We do not simply create skincare products.
✔ We deliver modern scientific skin regeneration solutions.

For product information, please contact
JN BEAUTY SOLUTIONS®
BEAUTY FROM SCIENCE